Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot:1:200-1:400 using ECL on rat brain membranes.
Immunohistochemstry on mouse brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Addition of 0.1-0.5% Tween-20 to the antibody solution is recommended.
Optimal working dilutions must be determined by the end user.

Anti-TrpC6 Antibody is an antibody against TrpC6 for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to amino acids 24-38 from mouse TRPC6 (Zhu et al. 1996). Accession Q61143

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes TRPC6 (Transient Receptor Potential-Like Channel 6, TRP-6). Does not cross react with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also work on human due to sequence conservation (15/16). Other species have not been tested.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.